- 期刊
Measurement of Mammary Epithelial Cell Size in Juvenile Macromastic Breasts
青春期巨乳症之乳房上皮細胞尺寸測量
摘要
巨乳症病患經常遭受身體與心理的不便，因而需要尋求手術改善。巨乳症依其乳房異常增大的時期，可分為青春期巨乳症與懷孕期巨乳症。巨乳症的成因可能是由於青春期或懷孕期乳房發育基因的表現異常而造成，但目前的研究對此並沒有很詳細的研究，與巨乳症相關的研究大多集中於病患的心理生理評估及手術方法的比較，乳房發育的相關研究目前都集中於乳癌與泌乳退乳的機制。器官的尺寸與細胞的尺寸及生長有關，因此本研究旨在量測青春期巨乳症病患乳房組織上皮細胞的尺寸，以釐清巨乳症成因與乳房上皮細胞尺寸的關係。 我們蒐集西元2003年至2005年因青春期巨乳症而至台大醫院接受縮乳手術且乳房尺寸大於等於E罩杯的病患為巨乳組，對照組則選取同一時期因乳癌於臺大醫院接受乳房切除手術且乳房尺寸小於等於C罩杯的病患，選取其未受乳癌侵犯的乳房組織。排除的因素包括年紀大於29歲或小於18歲、過去曾有懷孕的記錄、月經不規則、BMI大於27、術前三個月內曾有荷爾蒙的使用、或不正常的術前荷爾蒙檢查。切下的乳房組織以福馬林固定製作成為石臘切片，並以hematoxylin及eosin染色。我們以此切片來量測乳房上皮細胞的尺寸。兩組的統計比較則使用Stata 5.0統計軟體來進行Wilcoxan rank sum test。 三年內共蒐集到八位巨乳症病患及五位對照組，兩組的乳房上皮細胞尺寸以Wilcoxan rank sum test比較，並沒有統計學上的意義(72.7±12.3μm^2 vs. 69.5±10.0μm^2, p=0.46)。 本研究發現青春期巨乳症的乳房組織與乳癌病患未受癌症感染之乳房組織，在上皮細胞之尺寸方面並沒有差異，青春期巨乳症的成因可能是與乳房上皮細胞之生長較為相關。
關鍵字
無資料並列摘要
Patients with macromastia not only suffer from physical discomforts but also psychosocial adjustment disorders and seek for surgical inter vention. Macromastia is divided into two groups, juvenile and gravid hypertrophy, according to the onset of excessive breast development. The cause of macromastia is not well understood and may be related to the mistakes in gene expression during puberty or pregnancy. There are a lot of researches focusing on the molecular mechanism of breast cancer and lactation, but seldom on macromastia. The size of an organ is determined by the size of the cells and the number of the cells, which depends on cell division and cell death. This study intended to investigate the difference between breast tissues from juvenile macromastia patients and non-tumor invaded breast tissue taken from breast cancer patients, by analyzing mammary epithelial cell size. Patients who received reduction mammaplasty for juvenile macr omastia in NTUH from January 2003 to December 2005 are enrolled as macromastia group. The control group includes the patients who received breast operation for breast cancer in NTUH in the same time period, with breast sizes not larger than C cup. The exclusion criteria are age over 29 or less than 18, positive pregnancy history, irregular menstral cycle, body mass index over 27, hormone exposure within 3 months, and abnormal hormone level. Sections (5 μm) from formalinμ-fixed, paraffin-embedded blocks were cut for each case, followed by hematoxylin & eosin stain. The sections were used for measurement of mammary epithelial cell size. Nonparametric Wilcoxan rank sum test was used for comparison of data among the two groups. There were 8 patients in the macromastia group and 5 patients in the control one. Mammary epithelial cell size measurement showed no difference between the two groups (72.7±12.3 μm^2 vs. 69.5±10.0 μm^2, p=0.46). The study showed no difference between the juvenile macromastic breast and non-tumor part of cancer breast in mammary epithelial cells, suggesting that the cause of juvenile macromastia may be from factors other than abnormal mammary epithelial cell size.