- 期刊
The Effects of Hyaluronic Acid and Glucosamine on the Migration and Proliferation of Tenocytes
玻尿酸與葡萄糖胺對肌腱細胞移行與増生的影響
摘要
肌腱斷裂或是病變是運動醫學中常見的疾病。已經發表的研究指出注射玻尿酸（hyaluronic acid）或口服葡萄糖胺（glucosamine）對於肌腱的修復有助益。肌腱的修復需要肌腱細胞（tenocyte）移行（migration）至受損處，而後肌腱細胞會增生（proliferation）與合成細胞外基質來幫助修復。然而玻尿酸或葡萄糖胺對肌腱細胞的影響至今仍然不明。本實驗使用來自於大鼠阿基里斯腱（Achillis tendon）的肌腱細胞。在肌腱細胞中加入不同濃度的玻尿酸與葡萄糖胺，再使用細胞穿透移行分析法（transwell filter migration assay）來評估肌腱細胞的移行能力；另外使用細胞存活率分析法（3-[4,5-Dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide (MTT) assay）來評估肌腱細胞的增生。細胞穿透移行分析法顯示玻尿酸不會影響肌腱細胞的移行，而葡萄糖胺則會使肌腱細胞的移行率下降（p＜0.05）。細胞存活率分析法顯示玻尿酸濃度增加與葡萄糖胺濃度增加皆使肌腱細胞的增生率下降（p＜0.05）。本研究結果顯示玻尿酸與葡萄糖胺都會抑制肌腱細胞增生。此外，葡萄糖胺甚至會抑制肌腱細胞移行。然而，玻尿酸與葡萄糖胺在肌腱修復的實際生理效益還需以動物實驗進一步驗證。
並列摘要
Tendinopathy or tendon rupture is a common disease. Studies have showed that hyaluronic acid and glucosamine benefit the process of tendon repair. Tendon healing requires the migration of tenocytes to the repair site, followed by the proliferation and synthesis of the extracellular matrix. However, the effects of hyaluronic acid and glucosamine on tenocytes remain to be explored. Here, we treated tenocytes from rat Achilles tendons with hyaluronic acid or glucosamine. The ability of tenocytes to migrate and proliferate was assessed by transwell filter migration assay and MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide) assay, respectively. Results from transwell filter migration assays showed that hyaluronic acid has no significant effect on tenocyte migration, whereas glucosamine can inhibit tenocyte migration. The results of MTT assays revealed that tenocyte proliferation decreases after hyaluronic acid or glucosamine treatment. In conclusion, hyaluronic acid and glucosamine inhibit tenocyte proliferation. Moreover, glucosamine inhibits tenocyte migration. The in vivo effect of hyaluronic acid and glucosamine on tendon healing needs to be further assessed.