- 期刊
- OpenAccess
A Rapid Method to Establish Suspension Cultures of Paulownia Species
建立泡桐屬懸浮細胞培養的快速方法
摘要
將泡桐、白桐、日本泡桐與台灣泡桐種子苗芽體或組培苗葉片直接培養在液體培養，在1 mo內即可建立懸浮培養。培養液爲MS培養基添加1 mg/L 2,4-D與0.1 mg/L kinetin，在暗室內置於100rpm轉速的迴轉式震盪器培養。培植體200 mg培養1-2 wk後，可誘導出4-5×10^5 cells/mL，且70-95%的細胞都具有活性。日本泡桐與台灣泡桐的定期繼代培養下可維持一年以上，但泡桐在不斷繼代培養後細胞逐漸喪失活力而失敗。日本泡桐細胞培養液與誘導懸浮細胞培養組成相同，但台灣泡桐細胞培養液則需降低2，4-D與kinetin濃度，並添加NAA與BA。此一細胞培養方法比一般需要癒合組織誘導階段的方法有效且省時，在固體培養基篩選鬆軟癒合組織做爲細胞培養的材料的步驟完全不需要。
並列摘要
Cell suspensions were obtained within one month by culturing seedling shoots or plantlet leaves of Paulownia fortunei, P. kawakamii, P. tomentosa and P. taiwaniana in liquid media. MS medium supplemented with 1 mg/L2,4-D and 0.1 mg/Lkinetin was used. Cultures were kept in the dark on a gyratory shaker at 100 rpm. Cells with densities of 4.5 10^5 cells/mL were released directly from 200-mg explants cultured within 1-2 wk. Most cells (70-95%) were viable. Cultures of P. tomentosa and P. taiwaniana were maintained for more than one year by regularly subculturing, while P. fortunei and P.kawakamii failed after successive culturing. The same medium was used for the subculture of P. tomentosa cells, but for the subculture of P. taiwaniana, are duction in both 2,4-D and kinetin concentrations coupled with the addition of NAA and BA was necessary . This method is effective and timesaving compared to the generally accepted method using callus tissue. The production of friable calluses on agar suitable for suspension culture was eliminated.