山葵(Wasabia japonica Matsum)之根腐病於2015年7月在桃園市復興區首次發現,病徵為根基部及葉柄基部水浸狀軟化及腐爛、最後植株葉片下垂、萎凋死亡,部分發病根莖及葉柄上產生白色黴狀物及橘紅色的子囊殼。經組織分離、形態鑑定及分子鑑定,發現病原菌為Nectria haematococca,將純培養的病原菌菌落以均質機做成菌泥浸泡根部接種,可產生與自然界相同的病徵,發病率為55.6%,而自人工接種的發病株上亦可以分離出與接種相同的病原菌,完成柯霍氏法則。實驗中初步篩選出殺菌劑腐絕、三得芬、貝芬替、免賴得及土肉桂葉片、檸檬香茅葉片及樟樹葉片的精油,加入PDA培養基內對菌絲生長均有良好的抑制效果。
Root rot of Wasabia japonica caused by Nectria haematococca was first observed in Fuxing District of Taoyuan City in July, 2015. Diseased plants showed stem and rhizome rot, and subsequently leaves wilted and the plants died. Nectria haematococca was isolated from infected tissues, and the disease was reproduced in the lab by inoculation of the roots with a mycelial and spore suspension of the pure pathogen culture. After inoculation, 55.6% of plants developed the same stem and rhizome rot symptoms, and the pathogen was reisolated from inoculated plants to complete the pathogenicity test. Amendment with the pesticides Thiabendazole, Tridemorph, Benomyl, or Carbendazim, or essential oils of Cinnamomum osmophloeum, Cymbopogon citrates and Cinnamomum camphora leaves into potato dextrose agar medium, effectively inhibited mycelial growth of N. haematococca.