- 期刊
Application of Allele-Specific Polymerase Chain Reaction for Rapid Differentiation of Mycobacterium Leprae from Mycobacterium Tuberculosis
使用對偶基因特異性聚合連鎖反應快速辨別麻風分枝桿菌及結核分枝桿菌感染
摘要
麻風（漢生病）是一種由麻風分枝桿菌所引起之慢性肉芽性感染，主要侵犯皮膚及神經。麻風是一種重要的臨床課題，對於臨床醫師而言此疾病的診斷也是一項挑戰。有時麻風與結核菌感染之尋常性狼瘡不易做鑑別診斷，特別當感染發生於臉部時。我們報告一位70歲女性在左側眼部周圍有界限明顯逐漸增大的紅色斑塊，疑似麻風或尋常性狼瘡。在皮膚切片及施行對偶基因特異性聚合連鎖反應後確認麻風的診斷。使用對偶基因特異性聚合連鎖反應可以快速正確鑑別診斷麻風及結核感染。
關鍵字
無資料並列摘要
Leprosy (Hansen's disease) is a chronic granulomatous infection caused by Mycobacterium leprae affecting primarily skin and nerves. Leprosy is an important clinical problem and is also a challenging diagnostic problem for clinicians. Clinically, leprosy might at times be difficult to be distinguished from lupus vulgaris. We report a 70-year-old woman who showed a well-demarcated erythematous enlarging plaque on left peri-ocular region suggesting leprosy resembling lupus vulgaris. Skin biopsy was performed and mycobacterial infection was identified. However, it was uncertain to differentiate between tuberculosis and leprosy. To solve the problem, we design an allele-specific polymerase chain reaction based on two-nucleotide difference between bacilli of TB and leprosy. Our result showed allele-specific polymerase chain reaction can rapidly differentiate the infection caused by M leprae or by M tuberculosis.