- 期刊
微脂粒包埋不同濃度的四環黴素對於造骨細胞增生的影響
The Effect of Tetracycline Embedded Liposomes on the Proliferation of Rat Osteoblast-Enriched Cultures
摘要
促進牙周組織的再生，是牙周治療的最終目標，而造骨細胞會參與骨質的分泌和鈣化，所以對新骨的形成扮演著重要的角色；微脂粒爲人工合成具有雙層磷酸脂分子，可以形成鈣化的核心；另外四環黴素常用來作非手術性的牙周治療，也可以抑制宿主產生膠原蛋白酶來破壞牙周組織，希望可以利用各別的優點，對於新骨的形成可以有最佳的效果。造骨細胞取自Sprague-Dawley品種大白鼠的胚胎初代培養的細胞，利用負電荷磷酸脂絲氨微脂粒(egg phosphatidylcholine cholesterol, bovine brain phosphatidylserine)包埋在不同濃度的四環黴素(0, 0.065, 0.13, 0.26μg/ml)，從計算細胞培養24hr、48hr、72hr、96hr後的結果來比較對細胞增生的影響。結果顯示微脂粒包埋不同濃度的四環黴素，對於造骨細胞的細胞增生沒有顯著的影響，因此微脂粒包埋此濃度範圍的的四環黴素是不會影響造骨細胞的生長。
並列摘要
The major aim of therapies for bony defects is promoting truly regeneration. Osteoblasts, differentiated from primitive mesenchymal cells, play an important role in bone formation, including cell proliferation, synthesis, bone matrix secretion and calcification. Liposomes which enriched in cell-dervied matrix vesicles serve as nuclei for new crystals formation. They are useful membrane models for the study of biological mineralization. Tetracycline is a useful therapeutic agent in periodontal treatment, which may have influence on the process of new bone formation. The purpose of the experiment was to observe the proliferation of osteoblast-like cells by measuring cell numbers. The dose dependency and time effect of liposomes on cell number were analyzed. Effects on cell proliferation in embedded and nonembeded groups were not significantly different. These findings show tetracyclines embedded liposomes do not reduce the expression of osteoblast-like cells or the cell growth in primary rat osteoblast-enriched cultures.