Purpose: The trend of Objective Structure Clinical Examination (OSCE) adoption in pharmacy education is increasing. The study aimed to examine pharmacists' perceptions of OSCE training. Two targeted areas were: (a) pharmacists' cognitive attitudes toward OSCE educational training, and (b) directors' perceptive on pharmacists' professional skills after pharmacists received OSCE educational training. Methods: The study adopted a survey research methodology to collect related data. An observation questionnaire for the pharmacist' director and a self-reported questionnaire for the pharmacist were developed. Through research campaign, 25 directors and 72 pharmacists completed the survey. The descriptive statistics and Kruskal-Wallis test were used for analyzing the data. Results: Overall, pharmacists' cognitive attitudes toward OSCE educational training remained positive. The level of cognitive attitude presented a medium-high status. In addition, pharmacists' directors demonstrated a high level of satisfaction toward OSCE training. However, background information might not server as a variable to influence pharmacists' cognitive attitudes and the observation of pharmacists' directors. Conclusions: Regardless of the type of background information, from a trainee perspective, the study confirmed that OSCE training might benefit in-service pharmacists' professional development; from an employer perspective, OSCE training might be a valuable investment to effectively increase employees' skills and meet hospitals' need.