並列摘要
Because 'Szu Chi' green onion has been vegetatively propagated for a long period in Taiwan, the vegetative clones are susceptible to virus infection. The quality and yield have been decreased over the years. For developing the technology of mass production of virus-free green onion seedlings for farmers, the purpose of this study was to investigate the growth conditions for shoot tip tissue culture, determine the appropriate media and cultural conditions for shoot tip and plantlets regeneration. The shoot tips of three 'Szu Chi' green onion cultivars (lines), namely 'Lan Yang No.1', 'Tao Yuan No.3', and 'Hsin Chu 821', were cut and cultivated in four solid culture media: BP, BT1, BT2, and BT3. The results indicated that BP was the most suitable medium for shoot tip propagation; not only the survival rate, but also the number of regenerated shoots was great. The regenerated shoots were then transferred into the BP medium with addition of 0.1 ppm NAA could optimize the rate of root regeneration. The survival trate of in vitro plantlets of 'Tao Yuan No.3' and 'Lan Yang No.1' was 40% and 50%, respectively.