Background and Purpose: Around 60% of stroke patients have difficulty walking, leading to reduced quality of life. Metronome-based gait training uses rhythmic auditory stimulation to facilitate movements. The purpose of this study was to investigate the effects of metronome-based gait training on gait parameters in chronic stroke patients. Methods: A search was conducted in 3 databases (PubMed, Cochrane, Physiotherapy Evidence Database scale [PEDro]) for randomized controlled trials published before May 1, 2023. The methodological quality was assessed by PEDro and Oxford Centre for Evidence-based Medicine. Meta-analysis was conducted using Review Manager 5.4.1. Results: A total of 7 studies were included for meta-analysis. Compared with control group, the results showed a significant increase in gait speed (mean difference [MD]; 95% confidence interval [CI]) (MD = 0.12 m/s; 95% CI: 0.04 to 0.19; p = 0.002) and stride length (MD = 0.15 m; 95% CI: 0.12 to 0.19; p = 0.00001) in chronic stroke patients after metronome-based gait training. However, there was no significant improvement in cadence (MD = 6.23 step/ min; 95% CI: -0.68 to 13.15; p = 0.08) and Timed up & go test (MD = 1.53 sec; 95% CI: -1.79 to 4.86; p = 0.37). The subgroup analysis showed a significant increase in gait speed (MD = 0.12 m/s; 95% CI: 0.05 to 0.19; p = 0.0004) after metronome with treadmill training; while there was no significant increase in gait speed (MD = 0.10 m/s; 95% CI: -0.03 to 0.23; p = 0.14) after metronome with overground training in chronic stroke patients. Conclusions: The available evidence suggests that metronome-based treadmill gait training significantly improved gait speed for chronic stroke patients. Clinical Relevance: In the future, the metronome can be added to the treadmill training to optimize the gait function of chronic stroke patients.