- 期刊
高氏柴胡製備小柴胡湯之生物活性成分與抗氧化功能
Antioxidant Activities and Bioactive Compounds of Xiao-Chai-Hu-Tang Prepared with Bupleurum kaoi
摘要
小柴胡湯是以柴胡為主藥配合半夏、人參、紅棗、黃芩、甘草及乾薑等六種中藥材組成，小柴胡湯具有多種生理功效，更是治療慢性肝炎之重要中藥處方。北柴胡(Bupleurum chinense DC)進口自中國大陸，是最常使用的柴胡品種；而高氏柴胡(B. kaoi Liu, Chao et Chuang)為台灣特有原生種藥用植物，雖單方經研究證實具有免疫調節、保肝、抗發炎等多種效用，用於複方小柴胡湯中之抗氧化功能卻少有研究報導。本研究利用高氏柴胡與北柴胡配製小柴胡湯，分析水萃取物中之柴胡皂素、黃芩苷、黃芩素及甘草酸等含量，並配合抗氧化活性測定，以評估利用高氏柴胡取代北柴胡配製小柴胡湯之可行性。試驗結果得知，各萃取物之抗氧化能力與濃度呈現量效關係(dose-dependent relationship)。北柴胡雖含較多量的總酚與類黃酮化合物，但是高氏柴胡製備的小柴胡湯在清除DPPH自由基、還原力及總抗氧化能力等方面具有與北柴胡製備的小柴胡湯相當之效能。由本試驗結果顯示，高氏柴胡可替代北柴胡作為中藥複方之藥材。
並列摘要
Xiao-Chai-Hu-Tang (XCHT), an important prescription for curing chronic hepatitis in Chinense herbal medicine, is formulated with Bupleurum as the major ingredient together with Pinelliae tuber, Ginseng radix, Zizyphi fructus, Scutellariae radix, Glycyrrhizae radix, and Zingiberis rhizome. While Bupleurum chinense DC is imported from Mainland China and is the most commonly used species of Bupleurum, B kaoi Liu, Chao et Chuang is the aboriginal species in Taiwan. Although B. kaoi has been reported to possess the immuno-regulatory, hepato-protective and anti-inflammatory capabilities, its functionalities when used in compound prescription such as XCHT are rather scarce in the literature. In this study, both B chinense and B. kaoi were used to formulate XCHT and the water extracts of the resulting XCHT were prepared and analyzed for their antioxidant components and antioxidant functionalities to investigate the feasibility of replacing B. chinense with B. kaoi. In general, the dose-dependent relationship was found between antioxidant functionalities and the concentration of B. chinense, B. kaoi or XCHT extracts. Although B. chinense contains higher amounts of total phenolics and flavonoids, B. kaui-containing XCHT exhibited same levels of DPPH radical scavenging effect, reducing power, and total antioxidant capacity as B. chinense-containing XCHT In conclusion, B. kaoi can be used as a substitute for B. chinense in the compound herbal prescriptions.