- 期刊
水稻癒合組織之植株再生能力與澱粉代謝的關係
The Regenerability of Rice Callus is Closely Related to Starch Metabolism
摘要
本試驗以水稻矮南早39(ANT39)為材料，觀察在癒合組織誘導與植株再生過程中，澱粉代謝相關酵素如ADP glucose pyrophosphorylase、starch phosphorylase，與α-amylase之表現與組織定位。結果顯示具高植株再生能力的ANT39-Dry在癒合組織誘導過程中有較高澱粉與葡萄糖累積，在植株再生初期也有較高葡萄糖累積的現象。進一步分析顯示高澱粉含量主要是因為具有較高之澱粉生合成能力，而植株再生初期較高葡萄糖含量來自於培養基吸收與細胞內澱粉分解。此外，不論是starch phosphorylase或α-amylase表現位置均與澱粉粒分布及細胞分化之位置有密切相關，由上述結果推測澱粉代謝與水稻癒合組織再生植株有關，甚值得進一步探討。
並列摘要
To determine the relationship between starch metabolism and shoot regeneration in rice (Oryza sativa L. cv. Ai-Nan-Tsao 39, ANT39) callus, the distribution of starch phosphorylase and α-amylase in rice during callus induction and shoot regeneration were studied by immunohistochemical analysis. The results showed that highly regenerable callus, ANT39-Dry, possesses higher starch content at callus induction stage and higher glucose content at shoot regeneration stage than lower regenerable callus, ANT39-Wet. The higher starch content mainly caused from higher ADP glucose pyrophosphorylase activity. Besides, the higher glucose content at the initial regenerative stage possible resulted from medium and/or cellular starch and sucrose degradation. In addition, we found the localization of starch phosphorylase and α-amylase were closely related to starch grains especially at the peripheral region of callus where is putative the regeneration site. It suggested that starch metabolism might play a critical role at callus induction and shoot regeneration.