- 期刊
攝食添加Bifidobacterium longum BB536鮮乳對人體腸道菌相及其糞便萃取液對Caco-2及HT-29細胞株毒性之影響
Effects of Fresh Milk plus Bifidobacterium longum BB536 Intake on Human Intestinal Microflora and Cytotoxicity of Fecal Extracts on Caco-2 and HT-29 Cell Lines
摘要
The microflora of human gastrointestinal tract is a very complex ecosystem; the balance of beneficial bacteria bifidobacteria and harmful bacteria Clostridium perfringens is highly related to human health. This study was conducted to investigate the effects of fresh milk plus Bifidobacterium longum BB536 intake on the changes of intestinal microflora, fecal water content, and pH values in 24 healthy adults during 4 weeks of experiment periods and their fecal extracts cytotoxicity on Caco-2 and HT-29 cell lines. This experiment was divided into 4 periods, i.e. dietary regulated period (period 1), fresh milk plus B. longum BB536 intake period (period 2 and 3) and washed out period (period 4). Results indicated that the fresh milk plus B. longum BB536 intake significantly increased the colony counts of fecal bifidobacteria (p＜0.05) and decreased the growth of C. perfringens (p＜0.05). Subjects' height, weight, BMI, blood parameters, fecal water content and fecal pH did not significantly change during experimental period. Each period of subjects' fecal extracts at low concentrations showed no cytotoxicity on Caco-2 and HT-29. The cytotoxicity of subjects' fecal extracts at high concentrations on Caco-2 and HT-29 were significantly reduced during fresh milk plus B. longum BB536 intake periods (p＜0.05).
並列摘要
The microflora of human gastrointestinal tract is a very complex ecosystem; the balance of beneficial bacteria bifidobacteria and harmful bacteria Clostridium perfringens is highly related to human health. This study was conducted to investigate the effects of fresh milk plus Bifidobacterium longum BB536 intake on the changes of intestinal microflora, fecal water content, and pH values in 24 healthy adults during 4 weeks of experiment periods and their fecal extracts cytotoxicity on Caco-2 and HT-29 cell lines. This experiment was divided into 4 periods, i.e. dietary regulated period (period 1), fresh milk plus B. longum BB536 intake period (period 2 and 3) and washed out period (period 4). Results indicated that the fresh milk plus B. longum BB536 intake significantly increased the colony counts of fecal bifidobacteria (p＜0.05) and decreased the growth of C. perfringens (p＜0.05). Subjects' height, weight, BMI, blood parameters, fecal water content and fecal pH did not significantly change during experimental period. Each period of subjects' fecal extracts at low concentrations showed no cytotoxicity on Caco-2 and HT-29. The cytotoxicity of subjects' fecal extracts at high concentrations on Caco-2 and HT-29 were significantly reduced during fresh milk plus B. longum BB536 intake periods (p＜0.05).