- 期刊
廣藿香抗氧化成分之分離純化及結構鑑定
Separation, Isolation and Identification of Antioxidative Components from Pogostemon chablin
摘要
本研究探討廣藿香（Pogostemon chablin）之抗氧化成分，廣藿香粉末依序以正己烷、乙酸乙酯及甲醇萃取、過濾、濃縮後檢測其抗氧化性，三種溶劑萃取物之抗氧化性依序為BHA＞甲醇萃取物＞α-生育醇＞乙酸乙酯萃取物＞正己烷萃取物＞控制組。選擇具抗氧化較佳之廣藿香甲醇萃取物，經XAD-7液相層析區分出顯現強抗氧化性的沖提部分，利用高效液相層析法分析，選擇具抗氧化性，且所含成分較不複雜之區分，重複以高效液相層析分離純化，藉各種光譜分析法鑑定，得到三種純化物質，分別為adenosine、4-hydroxyethylcatachol-1-O-（2'-α-L-rhamnopyranosyl）-β-D-glucopyranoside及ο-coumaroyl-β-D-glucopyranoside。
並列摘要
The purpose of this study was to investigate the antioxidative components from Pogostemon chablin. The ground powder of Pogostemon chablin was sequentially extracted with n-hexane, ethyl acetate and methanol. The antioxidative activity of the extract was measured by the ferric thiocyanate method. The order of antioxidative activity was: BHA ＞ methanol extract ＞ α-tocopherol ＞ ethyl acetate extract ＞ n-hexane ＞ control. Due to the strong antioxidative activity of methanol extract, it was fractionated and isolated by various chromatographic method to obtain three pure components identified as adenosine, 4-hydroxyethyl-catachol-1-O-(2'-α-L-rhamnopyranosyl)-β-D-glucopyranoside, ο-coumaroyl-β-D-glucopyranoside.