- 期刊
茯苓固態發酵產物之降血糖的研究
Study on Hypoglycemic Activities of ＂Poria cocos＂ Solid-state Fermented Products
摘要
茯苓為一種食藥用其菌，主要生物活性成分為三萜類及茯苓多醣體。藥理研究證實其具有抗發炎、抗氧化、免疫調節、抗腫瘤、降血糖、鎮靜、利尿和止吐等作用。本研究之目的為探討茯苓固態發酵過程中，產物中粗多醣和粗三萜的含量變化，及其粗萃物之降血糖功能。結果顯示茯苓固態發酵產物以發酵兩週的茯苓發酵糙米產物之粗多醣含量最多，發酵四週的茯苓大豆發酵產物之粗三萜含量最高。配製50＂ppm＂茯苓粗三萜萃取物於STZ小鼠的飲水中，會顯著降低飲水量、攝食量和空腹血糖值，但餵食800＂ppm＂茯苓粗多醣萃取液則無明顯降血糖效果。且茯苓發酵大豆乙醇萃取物會促進PCI2細胞中PPRE介導的報導基因表現活性，且有顯著性促進PPRE所謂節的基因表現，茯苓大豆乙醇粗萃取物的半致效應濃度（EC_(50)）為106＂ppm＂。
並列摘要
＂Poria cocos＂ is an edible and medicinal fungi whose main bioactive compounds are polysaccharides and triterpenoids. Pharmacological studies confirmed that it has several effects such as anti-inflammatory, antioxidant, immunomodulatory, anti-tumor, hypoglycemic, sedative, diuretic, and antiemetic. The objectives of this research was to analyze the change of crude polysaccharides and triterpenoids contents in products of ＂Poria cocos＂ from solid-state fermentation and to study the hypoglycemic effects of extracts from ＂Poria cocos＂ solid-state fermented products . The results showed that the highest crude polysaccharides and triterpenoids contents were in ＂Poria cocos＂ 2-weeks solid- state fermented brown rice and 4-weeks solid-state fermented soybean, respectively. Fifty ＂ppm＂ of ＂Poria cocos＂ crude triterpenoid extract was added in drinking water for STZ mice, and it significantly reduced the amount of food and water intake and glucose level. However, there was no significant hypoglycemic effect when 800 ＂ppm＂ crude polysaccharides extract was provided for STZ mice. The ethanol extract from ＂Poria cocos＂ solid-state fermented soybean significantly promoted PPRE regulated gene expression, and the EC_(50) ＂Poria cocos＂ soybean ethanol extract was 106 ＂ppm＂.