- 期刊
以基因晶片技術篩檢耐力型與速度型優秀運動員基因表現量之差異
Using Microarray to Screening the Different Gene Expression between Elite Endurance Athletes and Power Athletes
摘要
過去的研究證明耐力型與速度型頂尖運動員表現不同的遺傳特徵，肌肉切片技術合併組織化學實驗證明兩類運動員在肌肉纖維上有差異。針對這兩類型運動員尋找簡易的篩選與分類指標將有助於提昇運動競技成績。以往分類耐力型與速度型頂尖運動員遺傳特徵的技術是採用肌肉切片技術，以針頭直接穿透肌肉深層切取肌肉，此類技術侵入性相對於靜脈採血更高。這個技術上的缺點也使得此類型研究的結果受限於受試者參與之意願，而且僅能提供單一基因之特性。近年來發展出一套高效率的篩檢工具-基因晶片，一次可篩檢數千個基因，比對基因表現量之差異。因此，本研究的主要目的為使用基因晶片技術，以選手之血液樣本進行比較兩類頂尖運動員之間基因表現之差異。結果發現耐力型與速度型有44個基因表現量有差異。耐力型比速度型基因表現量有5個增加、24個降低和15個統計上有顯著差異的降低。這些結果顯示耐力型與速度型確實存在著不同的基因表現，並且提供一些新的資料有助於未來研究耐力型與速度型選手選材之參考。
並列摘要
Muscle biopsy technique combined with histochemistry technique has demonstrated that endurance athletes and power athletes are endowed with different genetic traits. This has provided direct evidence on the variability of their muscle fiber type. While muscle biopsy technique provides one aspect of the phenotype of athletes, it is relatively invasive in nature. Experimental results derived from this study do not easily provide data. Furthermore, the information provided is relatively simple. Therefore, having a technique for selecting specialized athletes is important to the purpose of athletic performance, as well as for the purpose of detecting their live-time sports activity. Recently, the microarray technique has been widely used to screen gene expression to detect several thousands of different gene expressions. Thus, the major purpose for using the gene chip technique is to identify gene expressions in endurance athletes and power athletes. The results revealed a difference of 44 gene expression between the two types of athletes. Endurance athletes/power athletes are 5 genes up-regulated, 24 genes down-regulated and 15 genes down-regulated that have significant difference. The data suggest that endurance athletes and power athletes have different gene expression. Knowing this helps us to study the difference between endurance athletes and power athletes