- 期刊
- OpenAccess
Cytotoxicity and Exposure Assessment of PM_(2.5) in a Residential Home during COVID-19 Lockdown
摘要
As individuals become more confined to their homes, especially during the COVID-19 lockdown and the post-pandemic era, human activities will continue to generate more indoor particles. However, the toxicity effects of indoor particles remain unknown during residents' occupancy time. Eighteen 24 hours of indoor and outdoor PM_(2.5) samples were collected using 37 mm polyvinyl chloride (PVC) filter within a residential terrace house in Serdang, Selangor, during the 2021 Malaysia lockdown between February and March 2021. PM_(2.5) samples were then extracted using methanol. MTT assay determined the cytotoxic activity of extracted indoor and outdoor PM_(2.5) treated at different concentrations (25-200 µg mL^(-1)) on human lung cells (MRC-5) at a 24-hour incubation period. The 24-h mass concentration of outdoor PM_(2.5) (41.4 ± 1.99 µg m^(-3)) was significantly three times higher than indoor PM_(2.5) (11.8 ± 0.60 µg m^(-3)) (p < 0.05). However, exposure to indoor PM_(2.5) at higher concentrations (100 and 200 µg mL^(-1)) on lung cells (MRC-5) significantly reduces cell viability compared to outdoor PM_(2.5), suggesting that exposure to indoor PM_(2.5) causes toxicity to the lung cells compared to outdoor PM_(2.5). In parallel, indoor real-time PM_(2.5) measurements were recorded in the kitchen during cooking and non-cooking days. We found cooking days generated higher indoor PM_(2.5) concentrations (maximum PM_(2.5) = 75.0 µg m^(-3)), suggesting that cooking activity might contribute to the toxicity of indoor PM_(2.5). Due to the limited yield of indoor and outdoor PM_(2.5), further optimization on the extraction of PM_(2.5) should be carried out to evaluate further the mechanism of cytotoxicity of indoor PM_(2.5) on the lung cells.