Objective: Observe the thrombolytic effect of urokinase in the treatment of thrombosed permanent catheter of dialysis patients. To provide clinical nursing experience by studying the effectiveness and safety of urokinase. Methods: Diluted Urokinase was instilled in thrombosed catheter for 50 patients with occluded permanent catheter between July 2009 and March 20I0 in the dialysis centre. The effect of thrombolytic treatment was observed. Results: 47 cases of thrombosed permanent catheter regained sufficient blood flow after treatment, 3 cases have not achieve the effects. No side effects of all 50 cases were observed. Conclusion: Intracatheter thrombolysis with Urokinase is effective safe and easy to perform in clinical practice with no obvious side effect. It is a good method to maintain an effective blood access among hemodialysis patients.