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2010年台灣部分雞隻家禽白血病J亞群之血清抗體盛行率

The Serologic Prevalence of Subgroup J Avian Leukosis Virus in Some Chickens in Taiwan in 2010

Abstracts


家禽白血病病毒(avian leukosis virus; ALV)為雞場中常見引起腫瘤性疾病與造成雞隻生產問題的病原。由於台灣的土雞並未進行任何ALV-J的清除撲滅計畫,因此本研究希望藉由血清學的調查,了解台灣的肉用種雞(broiler breeder; BB)與蛋用種雞(layer breeder; LB)於ALV-J的感染情形。實驗選用6個包含不同年齡、品系的蛋用肉用種雞之雞場,調查ALV-J抗體盛行率,採集血液樣本以商品化酵素標示免疫吸附分析套組之檢驗(ELISA kit)進行抗體檢測。蛋用種雞樣本中,結果顯示只有1雞群抗體檢測為陰性,其餘皆為陽性,雞群的抗體陽性盛行率從6%到86.7%不等;肉用種雞樣本檢驗皆為抗體陽性,抗體陽性盛行率從15%到97.8%不等。同時發現雞隻週齡越高,相對抗體的陽性盛行率也越高。另外收集蛋用種雞血液樣本感作具有ALV-J感受性的DF-1細胞,PCR檢測病毒核酸皆為陰性結果。結果顯示:目前台灣的肉用種雞與蛋用種雞仍然存在ALV-J感染的情形。

Parallel abstracts


Avian leukosis viruses (ALVs) are common in poultry flocks and the causes of neoplastic diseases and other reproductive problems in chickens. In Taiwan, the country chickens were not performed ALV-J clearance programs in the farms. Thus, the infections of ALV-J in broiler breeders (BBs) and layer breeders (LBs) are suspected. We surveyed 6 farms with different chicken ages, breeds and types to detect the ALV-J antibody (Ab) prevalence. Chicken serum and plasma were collected and tested with commercial ELISA kit. Among the farm of tested LBs, only one farm showed negative ALV-J Ab results. The positive rate of tested LBs ranged from 6% to 86.7%. And the tested BBs showed all positive ALV-J Ab results, the positive rate ranged from 15% to 97.8%. Moreover, the older the chickens were, the higher the positive rate found in the farms. The blood buffy coat collections of LBs for viral isolation were negative in ALV-J nucleic acid detection by PCR. According to the data, it demonstrated that ALV-J infection still existed in the farms of BB and LB in Taiwan.

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