- 期刊
應用光固化3D列印方法製作細菌天然色素之抗菌材料
Application of light-curing 3D printing technology to produce antibacterial materials with natural bacterial pigments
摘要
本研究利用光固化3D列印製作含細菌天然色素之抗菌材料。本研究選擇可產生細菌天然色素－靈菌紅素（Prodigiosin, PG）之微生物菌株Serratia marcescens。透過天然成分之增產培養基培養，後經萃取、濃縮等步驟，100mL培養基中可獲得1.817 mg PG。PG對革蘭氏陽性Staphylococcus aureus模式菌株具良好之抑菌率，經48小時可達96.1%，其最小抑菌濃度（Minimum Inhibitory Concentration, MIC）為2,270ppb。使用明膠－甲基丙烯（Gelatin methacrylate, Gel-MA）水凝膠、聚（乙二醇）二丙烯酸酯（polyethylene glycol diacrylate, PEGDA）及405nm特定波長之光固化劑VA086製成光敏型生物墨水（bio ink）。將其加入已知濃度PG，利用市售光固化3D列印機成功列印出PG-Gel-MA網格材料。對S. aureus進行XTT assay，發現此網格材料之短效期抗菌效果良好，反映時間1小時之抑菌率為76.9%。PG-Gel-MA 3D網格材料具溶脹率，其中添加2倍PG之3D網格材料在短時間達20.3±5.5%。本研究製作之材料未來可用於室內清淨空氣之抗菌濾材及皮膚傷口抗菌敷料等多種環境。
並列摘要
This study uses light-curing 3D printing technology, specifically photopolymerization, to produce antibacterial materials. The selected bacterial strain, Serratia marcescens, can specifically produce an antibacterial compound known as prodigiosin (PG), a natural red pigment found in bacteria. After incubation, a high purity of PG, amounting to 1.817 mg per 100 mL of modified medium, is obtained. The antibacterial ability of PG is significant; 2,270 ppb of PG presents an excellent inhibition rate of 94.92± 0.03% against the Gram-positive model strain, Staphylococcus aureus, during a 48-hour incubation period. The minimum inhibitory concentration (MIC) is calculated to be 1,720 ppb of PG. Furthermore, the bio-ink is composed of gelatin-methacrylate (Gel-MA) hydrogel, polyethylene glycol diacrylate (PEGDA), and an initiator (VA-086). A commercial 3D photopolymerization printer successfully printed an antibacterial grid containing PG-Gel-MA. The grid exhibits excellent antibacterial ability against S. aureus, presenting a superior antibacterial efficiency of 76.9% within just 1 hour. The antibacterial grid also exhibits a certain degree of swelling, with the swelling ratio calculated as 20.3±5.5% (2PG-Gel-MA) within 45 minutes. In the future, the 3D antibacterial grid can be applied in various conditions, such as serving as antibacterial filters for indoor air bioaerosol control and as antibacterial dressing gauze for skin wounds.