- 期刊
Evaluation of Animal Models of Neck Vessel Operation with 3D Time-of-flight MRA
以臨床造影儀之頸部時間飛行磁振血管造影評估動物模式之頸部血管手術成功與否
摘要
頸靜脈逆流與雙側總頸動脈永久阻塞之大白鼠動物模型已被廣泛應用於大腦動靜脈廔管、大腦靜脈高壓和慢性大腦血流灌注過低之研究。然而需要方法用以評估這些血管手術的成功與否。故本研究以臨床造影儀之時間飛行磁振血管造影術分別於頸靜脈逆流與雙側總頸動脈永久阻塞之大白鼠動物模型手術前後進行取像，併以最大強度投影重組得到高解析度之大鼠頸部血管影像。在頸靜脈逆流動物模型中，以動脈攝影上出現被逆流血液強化的靜脈血管構造評估手術的成功與否，影像亦提供血液逆流高度及其他頸部血管狀態的資訊。而雙側總頸動脈永久阻塞之大鼠模型中是以影像上缺乏兩側總頸動脈的血流訊號為準則，另外影像亦顯示頸部側枝血管的生成發展。總而言之，對於監測此兩模型之眾多大腦血管的血流，頸部三維時間飛行磁振血管造影術是一個有用且非侵入性的方法，其亦顯示頸靜脈逆流的高度、側枝循環的生成和長時期研究之血管隨著時間的變化，故其應該被考量用以評估這些模型。
並列摘要
Rat models of jugular venous reflux (JVR) and bilateral common carotid artery occlusion (BCCAO) are widely used in studies of cerebral arteriovenous fistula, cerebral venous hypertension and chronic cerebral hypoperfusion. However, methods to evaluate the effectiveness of the operation are needed. We performed neck 3D time-of-flight (TOF) magnetic resonance angiography (MRA) with a clinical scanner in rat models of JVR and BCCAO before and after operation. High-resolution arterial angiography of the rat neck was acquired with maximum intensity projection. In the JVR model, the success of the operation was validated by enhanced venous structures on MRA. The angiogram also provided information on the height of JVR and the status of other neck vessels. In the BCCAO model, bilateral carotid occlusion was confirmed on MRA in the absence of carotid flow signals. In addition to validation of the occlusion, the angiogram also showed the development of neck collateral vessels. In conclusion, neck 3D TOF MRA is a useful noninvasive method for monitoring blood flow over time in longitudinal studies in both animal models, which should be considered in evaluation of these animal models.