- 期刊
含天然蘆薈清潔劑之抑菌評估
Evaluation of Bacteriostatic Effect of an Aloe-containing Sanitizer
摘要
根據本草綱目記載，蘆薈本身無毒無害無副作用，發現其內所含的皂角甘(saponons)具有強力清潔與抗菌效力，爲一天然皂素；而安特拉歸農綜合體(anthraquinone complex)具有消炎、消腫、抑制細菌生長、止癢、止痛等功效。蘆薈已被應用在化妝品、清潔日用品、健康食品、外傷治療等領域。本研究參照美國及歐盟對清潔劑抗菌效果的檢測方法，評估含天然蘆薈清潔劑的抗菌效果。測試菌株包括，大腸桿菌(Escherichia coli)、金黃色葡萄球菌(staphylococcus aureus)、凝固桿菌(Bacillus coagulans)、克雷白氏肺炎桿菌(Klebsiella pneumoniae)、綠膿桿菌(Pseudomonas aeruginosa)、傷寒桿菌(Salmonella typhi)等六株，進行基本殺菌效果評估與含有機物及硬水條件下的殺菌效果評估，各菌株皆進行不同三天的檢測，每株每種條件各檢測30次，共進行900次的檢測，檢測結果利用MS-Excel 97版及SPSS ver10.0統計軟體進行資料分析及繪圖。清潔劑與各種測試菌，經過30次不同天檢測，不論第一階段試驗或第二階段試驗，其結果皆符合歐美國家食品級清潔劑的要求，30秒內減少10^5以上的微生物數量，可適用於食品業、乳製品業、罐頭業、蛋業及其他非食品業；其效果也符合醫院的環境清潔劑的要求，對E. coli、P. aeruginosa、S. aureus，5分鐘內減少10^6以上的微生物數量。
並列摘要
Aloe has been cited by centuries-old Chinese herbal text as a non-toxic substance after human consumption, and has many beneficial effects for the human health. A recent report has indicated that one of its components, saponon, has bacteriostatic, in addition to cleansing effects. Another component, anthroquinolone complex, has bee reported to suppress inflammation, tissue swelling, and bacterial growth. The US Food and Drug Administration has approved the use of aloe in cosmetics, detergents, health foods, and for treatment of injuries for more than 10 years. We studied bacteriostatic effect of an aloe-containing sanitizer employing test methods of the American Environmental Protection Agency (EPA) and European Committee for Standardization (CEN). Six bacterial strains tested included Escherichia coli, Staphylococcus aureus, Bacillus coagulans, Klebsiella pneumoniae, Psueodomonas aeruginosa, and Salmonella typhi. Each strain was tested 30 times in the bacteriostatic suspension test and phase two suspension test over 30 seconds, the latter being used to determine the effect in hard water and 0.3% bovine albumin solution. The result showed that there was reduction of 99.999%, or 5 logs, in the number of all bacterial strains within 30 seconds, meeting both EPA and CEN standards as a sanitizer. The product could be applied to cleansing of food-contact or non-food contact surfaces.