In this article, by applying the theory of "persistent self-cultivation and reverence for life as well as maintenance of physical and mental health in line with the Taoism theory" as the research topic, a detailed discussion was carried out concerning the historical origin, historical inheritance, and core concept of Dao medicine culture in Qingcheng. Meanwhile, a summary was provided for the historical origin and theoretical overview of Qingcheng Dao medicine culture which advocated "persistent self-cultivation and reverence for life in line with the Taoism theory". Then, a relatively comprehensive explanation was also made regarding relevant methods of "health preservation and conditioning", such as diet therapy, guidance and spells. The inheritance and development of the "Dao medicine culture" was prospected in the era of National Health. Furthermore, this study also introduced the excavation, inheritance and development of Qingcheng Dao medical skills by Mr. Li Jiuyun, the contemporary inheritor of Qingcheng Dao medical skills, and his disciples. On the basis of sorting out and summarizing the cultural system of Dao medical knowledge under the Taoism theory of "harmony between man and nature", the new medical system and core theoretical thoughts of Dao medical knowledge were pioneered in this research, so as to achieve inheritance and benefit all human beings.