玫瑰花萃取物美白、抗氧化及抑菌活性之研究

玫瑰（Rosarugosa）花中包括多酚（polyphenols）、類黃酮（flavonoids）、酚酸類（phenolic acid）、單寧酸（tannic acid）和多醣（polysaccharide）等成分。本研究以熱水萃取物（Rosa rugosa extracted with hot water, HWRR）、50%乙醇萃取物（Rosa rugosa extracted with 50% ethanol, EERR50）及95%乙醇萃取物（Rosa rugosa extracted with 95% ethanol, EERR95）評估玫瑰花萃取物之酪胺酸酶抑制作用、抗氧化及抑菌活性。結果顯示，EERR95的總酚含量最高，HWRR的總黃酮含量最高。EERR50有較佳之DPPH清除自由基能力，SC_(50)為0.33 mg/mL，而EERR95表現出較好的酪胺酸酶抑制IC_(50)，為0.12 mg/mL。使用稀釋測定抑菌能力，HWRR對四種菌株的最小抑菌濃度（minimal inhibitory concentration, MIC）為2.31至9.26 mg/mL；EERR50和EERR95對三種大腸桿菌、金黃色葡萄球菌和白色念珠菌之MIC分別為2.31 mg/mL、2.31 mg/mL和2.31至4.63 mg/mL，因綠膿桿菌對乙醇敏感，無法測得。這項研究顯示，玫瑰花具有體外酪胺酸酶抑制作用、抗氧化及抑菌活性。

關鍵字

玫瑰花萃取物； DPPH ；酪胺酸酶；抑菌

並列摘要

Biological compounds were found in Rosa rugose rosebuds including polyphenols, flavonoids, phenolic acids, tannic acid, and polysaccharides. In this study, the hot water extracts (HWRR), 50% ethanol extracts (EERR50), and 95% ethanol extracts (EERR95) were used to evaluate the tyrosinase inhibitory effect, antioxidant and antibacterial activity of rose flower extract. Results indicated that the highest total phenolic content was observed in the EERR95, while the highest total flavonoid content was in the HWRR. The EERR50 exhibited DPPH scavenging ability of SC_(50) of 0.33 mg/mL, while EERR95 showed better tyrosinase inhibition of IC_(50) of 0.12 mg/mL. Using the dilution assay, HWRR was exhibited a minimum inhibitory concentrations (MIC) of 2.31 to 9.26 mg/mL against all four strains. EERR50 and EERR95 was shown to have MIC of 2.31 mg/mL, 2.31 mg/mL and 2.31 to 4.63 mg/mL, respectively, against 3 strains of E. coli, Staphylococcus aureus and Candida albicans, only P. aeruginosa was sensitive to ethanol. This study has revealed that Rosa rugosa rosebuds possess in vitro tyrosinase inhibitory, antimicrobial and antioxidant activity.