Pulse transit time, which correlates with blood pressure, is measured between the electrocardiogram R-wave peak and 50% raising front level of a photoplethysmographic (PPG) signal. Registered PPG signal bandwidth may be shared by noise and therefore the signal raising front is undetectable. Electrocardiogram reference adaptive sum comb filter was used to extract the harmonic components of the PPG signal and suppress the noises between them. Averaging effect of the filter on the PPG signal was studied and adjustments were made. The influence of the comb filtered PPG signal on the measurement of pulse transit time was analysed.