- 期刊
Content of alkaloids in ornamental Papaver somniferum L. cultivars growing in Estonia
Alkaloidisisaldus Eestis kasvavate unimagunate (Papaver somniferum L.) ilusortides
摘要
The alkaloid content in industrial opium poppies (Papaver somniferum L.) has been studied by numerous authors, but not in ornamental cultivars. The aim of the study was to investigate the content of morphine, codeine, and papaverine in P. somniferum cultivars by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). The studied poppy capsules were either grown from the ornamental poppy seeds purchased from different vendors or were unknown cultivars from home gardens in Estonia, cultivated as ornamental poppies. All dried capsules samples (n = 34) contained on average 362 mg/100g of morphine, 25 mg/100g of codeine, and 29 mg/100g of papaverine as determined by CE. The content of morphine determined by HPLC was 152-676 mg/100g, which is a remarkably high concentration allowing potential illegal use of ornamental poppies. Both analytical methods demonstrated similar results. Hence, CE is a suitable alternative to HPLC for quantitative analysis of opium alkaloids as it simplifies the sample preparation procedure.
並列摘要
Unimaguna (Papaver somniferum L.) tööstuslike sortide alkaloidisisaldust on uuritud mitmete autorite poolt, dekoratiivsortide oma aga mitte. Käesoleva töö eesmärk oli analüüsida morfiini-, kodeiini- ja papaveriinisisaldust erinevates unimagunasortides, kasutades kõrgefektiivse vedelikkromatograafia (HPLC) ning kapillaarelektroforeesi (CE) meetodeid. Uuritud taimne materjal kasvatati kaubandusvõrgus saadaolevatest seemnetest või koguti mitmetest Eesti aedadest, kus neid kasvatati tundmatute ilusortidena. CE põhjal oli kõikide kuivatatud kuparde proovide (n = 34) keskmine morfiinisisaldus 362 mg/100 g, kodeiinisisaldus 25 mg/100 g ja papaveriinisisaldus 29 mg/100 g. HPLC järgi jäi morfiinisisaldus erinevates sortides vahemikku 152–676 mg/100 g. Nii suur morfiinisisaldus on märkimisväärne ja võimaldab potentsiaalselt unimaguna ilusortide illegaalset kasutamist. Tulemused olid mõlema analüütilise meetodiga sarnased. CE pakub HPLC-le tugevat alternatiivi, kuna proovide ettevalmistamine analüüsiks on viimasest lihtsam.