- 期刊
脊髓損傷鼠以五爪金英萃取物處理後肌細胞內脂肪含量變化
Intramyocellular Lipid Level Changes in Rats with Spinal Cord Injury after Tithonia Diversifolia Extract Treatment
摘要
目的：根據國外文獻報導五爪金英（Tithonia diversifolia）萃取物具抗發炎、抗氧化等療效。但其萃取物如何影響脊髓損傷（SCI）後所引起肌肉活動力的變化，則無人報導過。且由於肌細胞內脂肪（IMCL）的含量為動物體骨骼肌在活動時的能量來源，故本研究利用氫磁振頻譜（MRS）觀察誘發大鼠SCI後口服五爪金英乙醇萃取物（TDE）五天後，其股四頭肌IMCL的含量改變情形。方法：以Philips 3T磁振掃描儀以及所配備之點解析頻譜（PRESS）波序取得18隻SD大鼠股四頭肌之單體素1H-MRS，參數設定為TR 2000 ms、TE 40 ms。動物損傷模式為脊髓胸椎第五段處（T5）以50克動脈夾壓迫1分鐘；TDE 則取五爪金英地上莖、葉部份經乙醇浸泡萃取所得。大鼠SCI後是否誘導成功則利用擴散張量造影測量T5脊髓段神經纖維束損傷情形。結果：由於有無SCI的動物其肌酸（Cr）訊號均相當穩定，故可做為生物體內參考值。且結果顯示：SCI後的動物經服用TDE 5天後其股四頭肌的肌細胞其IMCL/Cr值在8週後顯著下降。結論：TDE可影響脊髓損傷後大鼠股四頭肌肌肉恢復期的能量代謝，故IMCL含量會減少。
並列摘要
Objectives: Pharmacological studies of Tithonia diversifolia have shown that it has anti-oxidation and anti-inflammatory activity. After spinal cord injury (SCI), reduced neuromuscular activity leads to atrophy in the muscles innervated below the level of the lesion, especially when muscle activity is reduced. However, the effect of Tithonia diversifolia extracts on the muscle activity after SCI has not yet been evaluated. Magnetic Resonance (MR) techniques were used in this study and Magnetic Resonance Spectroscopy (MRS) was able to measure in vivo the changes in muscle intramyocellular lipid (IMCL), which is an energy consumption indicator. Methods: A point-resolved spectroscopy (PRESS) sequence with following parameters, TR/TE=2000/40 ms, NSA=96, ROI=5×8×5 mm3, was applied to eighteen male Sprague-Dawley rats weighing 280–320 g in order to acquire the MR spectra. T5 laminectomy and compression (50g, 1 min) were applied to induce SCI. Treatment with Tithonia diversifolia ethanolic extracts (TDE) (50 mg/kg B. W. orally, 5 days) was undertaken after the rats had undergone SCI. The TDE was dissolved in olive oil as a vehicle. Diffusion Tensor Imaging (DTI) has applied to evaluate the damage level of the fiber tracts in spinal cord. Results: These results showed that TDE treatment decreased the IMCL/creatine ratio in the quadriceps at 8 weeks after SCI 8 when compared to rats that had not undergone TDE treatment. Creatine was used as the internal standard. Conclusion: TDE would seem to be useful treatment that increases muscle activity in spinal cord injury rats and that this occurs via IMCL consumption.