近年替代醫學興起,雷公根的用途及藥效廣受重視,然而臺灣目前雷公根供應以進口或野外採集為主,易發生來源不明及混用其他青草甚至農藥殘留等問題。本試驗以環境控制系統配合紅藍光發光二極體(light-emitting diode; LED)及不同氮濃度養液栽培雷公根,評估此人工栽培系統對雷公根生長及指標成分三萜含量的影響。試驗結果顯示,氮濃度維持在105 ppm對雷公根生長有最佳效果,超過此氮濃度反而抑制其生長及株重。總三萜含量方面,各種氮濃度養液處理之結果皆無差異,而和市售雷公根比較,環控栽培系統生產的雷公根其總三萜含量亦無差異,顯示人工栽培系統並未影響總三萜含量,但是否影響個別三萜含量或型態則待後續研究。本試驗以全人工控制的環境可生產無農藥及無病蟲害干擾的雷公根,經養液調整後可提高株重,且不影響植株總三萜含量,此結果或可用於後續相關研究及商業生產用途。
"Centella asiatica" has received much attention due to the development of alternative medicine recently. However, import and wild collection are two main supply sources of "Centella asiaticais" in Taiwan that bring about the possibilities of unclear in source, confusion with other herbs and pesticide contamination. In this experiment, "Centella asiatica" was cultured under controlled environment equipped with light emitting diodes (LEDs) as light source and provided with different nitrogen concentrations in nutrient solution so as to study their effects on the growth and total triterpenoid content of this species. Results showed that nitrogen concentration at 105 ppm had the maximum plant weight, and was reduced at the concentrations above this level. No significant difference of total triterpenoid content existed among treatments including commercial product, indicating that the artificial system built in this case did not change production of total triterpenoid in "Centella asiatica". As such, the controlled environmental can be considered to produce nontoxic and pesticide-free "Centella asiatica" in future research as well as in commercial production.