- 期刊
高濃度蔗糖提高小米癒合組織植株再生能力之研究
Study on High Concentration of Sucrose Treatment during Callus Induction Enhances Shoot Regeneration in Setaria italica (L.) P. Beauv.
摘要
本研究利用小米臺東8號成熟種子為材料，於MSD2培養基中添加3%或12%蔗糖濃度處理，觀察對癒合組織誘導影響及後續將其移至不同濃度kinetin與NAA搭配組合的再生培養基中之植株再生情形。試驗結果顯示在含3％或12%蔗糖的MSD2培養基中，癒合組織誘導率為99%及78%，癒合組織鮮重與乾重均有隨培養週數增加而增加的趨勢，惟高濃度蔗糖處理者癒合組織生長明顯趨緩，乾重在培養四週後則與3%蔗糖處理者無顯著差異。此外，高濃度蔗糖處理可顯著降低癒合組織中水分含量。另一方面，將上述所誘導4週之癒合組織移至植株再生培養基，結果顯示癒合組織誘導階段以3%蔗糖處理者，不論在哪一種植物生長調節劑組合下，初期僅有胚狀體形成，2週後開始有不定根形成，無任何植株再生。反之，癒合組織誘導階段以12%蔗糖處理者，移至同時含2 ppm kinetin與0.5 ppm NAA之培養基中4週後，有20％植株再生率，此癒合組織在其他濃度kinetin與NAA組合下，也只有不定根形成或癒合組織增殖，綜合上述，12%蔗糖處理有利於誘導體胚形成及後續植株再生的可能機制，仍須進一步探討。
並列摘要
Millet (Setaria italica (L.) P. Beauv. cv. Taitung No. 8) matured seeds were cultured on MSD2 callus induction medium containing 3% and 12% sucrose (represented as MSD2Suc3 and MSD2Suc12, respectively) to evaluate the callus induction and following shoot regeneration response. The callus induction rate is 99% and 78% in MSD2Suc3 and MSD2Suc12, respectively. Both the fresh weight and dry weight were increased alone with culture period in MSD2Suc3 and MSD2Suc12 callus, however, they are significant higher in MSD2Suc3 than in MSD2Suc12. Besides, the water content in MSD2Suc3 is also higher significantly than in MSD2Suc12 during callus induction. After 28 days of culture, calli were transferred to regeneration medium with different concentrations and combinations of kinetin (2, 4, 8 ppm) and NAA (0.5, 1, 2 ppm). There is no shoot regenerated in MSD2Suc3 callus cultured for 4 weeks no matter of regeneration medium and only some embryoids and adventitious roots formation. On the other hand, the shoot regeneration frequency increased to 20% when MSD2Suc12 callus were transferred to MSK2N0.5 regeneration medium. But, the other combinations of kinetin and NAA in MSD2Suc12 callus similar to the response in MSD2Suc3 have no shoots regenerated and only embryoids and adventitious roots formation after 2 weeks of culture. More investigation is necessary to clarify the possible mechanism of 12% sucrose was supplemented in callus induction medium to enhance embryogenic callus induction and following shoot regeneration.