- 期刊
- OpenAccess
絲瓜幾丁聚醣含量測定與分析
Analysis on the Luffa Gourd Chitosan Content
摘要
絲瓜是台灣夏季重要蔬菜之一，由於含有幾丁聚醣成分，是現今頗受矚目的天然高分子材料，具有保濕、抗菌等作用，廣泛應用在生醫、食品、生技等領域。因萃取自絲瓜的幾丁聚醣分子量還不確定及所使用的高效能液相層析儀偵測器敏感度等因素，而利用標準品配置不同濃度，以分光光度計制定檢量線以求得絲瓜萃取液中幾丁聚糖含量，其不須經高倍數稀釋，可降低實驗誤差，故本研究以呈色法來檢測不同品種絲瓜不同部位幾丁聚醣含量。結果顯示絲瓜幾丁聚醣含量在不同型態品種間呈顯著差異，參試稜角絲瓜幾丁聚醣平均含量高於圓筒絲瓜，品種間最高與最低含量可相差5.676mg g^(-1)，以品種‘美菱’幾丁聚醣含量最高。絲瓜不同部位幾丁聚醣含量差異更為顯著，以嫩葉中幾丁聚糖含量10.241mg g^(-1)最高，絲瓜露中含量0.884mg g^(-1)為最低，不同部位間差異達9.357mg g^(-1)。本研究顯示絲瓜葉片中含有豐富的幾丁聚醣，以稜角絲瓜品種‘美菱’嫩葉中含量最高，可進一步開發利用。
並列摘要
Luffa gourd is an important summer vegetable in Taiwan. The chitosan of luffa gourd is a natural high polymer material that attracts attention now. Numerous studies have demonstrated that chitosan and its derivatives have various biological activities and have been proposed for diverse applications in biomedical, food, biotechnology, pharmaceutical and agricultural fields. The objectives of this study are to evaluate analysis method and the chitosan content among various luffa gourd cultivars and different parts of tissues. We used color test and High Performance Liquid Chromatography (HPLC) those two methods measured the content of chitosan. Due to the molecular weight of chitosan extraction from luffa gourd was uncertain and HPLC's detector sensitivity might not be applied to this system. Therefore, this study used color test to measure the content of chitosan from different cultivars and parts of luffa gourds. There were significantly different in chitosan content of cultivars and parts of luffa gourd. The chitosan content of angle luffa gourd was higher than that of sponge luffa gourd. The chitosan content was 5.676 mg g^(-1) and 9.357 mg g^(-1) between the highest and the lowest cultivars and parts, respectively. Cultivar 'Miriam' of angle luffa gourd had the highest chitosan content. Compare the chitosan content of different parts of gourd showed the highest content was 10.241 mg g^(-1) in young leaves and the lowest content was 0.884 mg g^(-1) in xylem sap of luffa gourd. This study showed the possibility of chitosan production and potential use from luffa gourd.