探討小球藻最適化醱酵培養條件

本研究使用小球藻構造簡單，生長快速，油脂含量高，行光合作用，吸收CO2生成O2與油質，是相當有潛力的生質能源生產者，因此研究目的在小球藻菌醱酵培養條件探討，開發有可行性的生質能源與CO2減量，並同時生產高附加價值之生物化學品。本實驗主要探討溫度、氮源、營養劑、維他命、光照波長之顏色、透光度，對小球藻成長之影響為研究，並由實驗設計法中得到小球藻最佳培養條件。最佳培養條件為：尿素1ml、維他命1ml、營養劑1ml、海鹽30g、溫度23˚C、日光燈40W照射下，1 liter收集的小球藻為0.923g。在實驗室中順利使用5 liter發酵槽放大發酵培養出高密度小球藻。

關鍵字

小球藻；最佳培養條件；生質能源；營養劑

並列摘要

In this study, microalgae Chlorella sp., which has simple structure, grows fast, contains high contents on fat, runs photosynthesis, and produces oxygen and fat by absorbing carbon dioxide, is a considerably potential biomass energy producers. Therefore, the purpose of this research is explore the fermentation culture conditions of microalgae Chlorella sp., developing the feasible biomass energy and carbon dioxide reduction, and producing high value-added biochemical products. This experiment mainly discusses effects of temperature, source of nitrogen, nutrient, vitamin, the color of the light wavelength, and light transmittance on growth of microalgae Chlorella sp., and finding the optimal culture condition of microalgae Chlorella sp. through the various designs of experiments. The optimal culture conditions are as follows: adding 1 ml of Urea, vitamin, and nutrient, and 30 gram of sea salt under the condition of 23℃ and 40 watt irradiation of fluorescent lamps, and 0.923 g of microalgae Chlorella sp. is collected in one liter. This experiment successfully develops high density of microalgae Chlorella sp. by enlarging the fermentation in 5-liter fermentor.