一葉草之成分及抗氧化活性分析

Components Analysis and Antioxidant Activity of Ophioglossum Petiolatum Hook

廖云彗(Yun-Huei Liao) ； 林佳靜(Chia-Ching Lin) ； 黃文盈(Wen-Ying Huang)

《長庚科技學刊》 27期 (2017/12) Pp. 19-26

https://doi.org/10.6192/CGUST.201712_(27).4

摘要

本研究以超效能液相層析質譜儀分析一葉草之成分，利用0.5 % acetic acid、acetonitrile 與methanol，以流速0.2 mL/min 沖提40 分鐘，在254 nm 下進行，分析出4 個成分：Luteolin、Quercetin、3-methoxy quercetin 與Ophioglonol。並以總酚含量、還原能力、清除DPPH 自由基以及清除超氧陰離子能力測定一葉草之抗氧化力。實驗結果顯示，總酚含量相當12.37 ± 0.23 μg/mL gallic acid、清除DPPH 自由基能力IC_(50) 為1.58 mg/mL、清除超氧陰離子能力IC_(50) 為1.02 mg/mL，代表一葉草具有抗氧化力。

關鍵字

超高效能液相層析質譜儀 ； 一葉草 ； 清除超氧陰離子能力 ； 抗氧化

並列摘要

In this study, ultra performance liquid chromatography mass spectrometer was employed to analyze the components of Ophioglossum petiolatum Hook. The mobile phases, 0.5 % acetic acid、acetonitrile、 methanol, were applied at a flow rate of 0.2 mL/min for 40 minutes and 254 nm. Liquid chromatography subseqently evealed four components, namely Luteolin、Quercetin、3-methoxy quercetin、 Ophioglonol. Furthermore, the total phenol content, reducing power, DPPH free radical scavenging activity, and superoxide anion scavenging capacity were obtained to determine the antioxidant activity of O. petiolatum . The results revealed that total phenolic content of O. petiolatum was equivalent to 12.37 ± 0.23 μg/mL gallic acid, IC_(50) value of DPPH scavenging activity was 1.58 mg/mL, and IC_(50) of superoxide anion scavenging capacity was 1.02 mg/mL.

並列關鍵字

ultra performance liquid chromatography mass spectrometer ； ophioglossum petiolatum ； superoxide anion scavenging capacity ； antioxidant

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