A sigmoidal transfer function with Mode Factor (MF) was developed to consider the fatigue life transition from constant strain mode to constant stress, or intermediate mode. The transfer function was combined with a laboratory fatigue prediction model which was developed under 618 constant strain four point bending fatigue tests to form a new asphalt pavement fatigue cracking prediction model. Subsequently, the model was calibrated with 26 full-scale accelerated and real pavement test sections. The calibration results indicate that the proposed model has achieved better prediction than the Asphalt Institute (AI) MS-1 fatigue prediction model in the most common region for the real pavement design life. This model can be an option for Mechanistic-Empirical pavement design and further refined upon verification.