In order to investigate effect of different concentrations of iron chelate Nano fertilizer on growth and performance of two kinds of Spinach, an experiment was performed as a plan of completely random plot in three alternations and in study field of Khazra Company in 2012. First studied factor was different types of Spinach including Varamin 88 and Viroflayand second studied factor was different concentrations of fertilizer (0, 2 and 4 ppt, respectively). Nano fertilizer solution injection was performed in early morning when plant had 8-13 leaves. Results show that wet weight and maximum leaf area index is influenced by concentration of iron chelate Nano fertilizer and dry weight is influenced by both type of Spinach and concentration of Nano fertilizer. Using 4 kg/ha Nano fertilizer causes 58 and 47% increase inwet weight and maximum leaf surface index, respectively comparing to use of no fertilizer. Using 4 kg/ha Nano fertilized leaded to maximum value of aerial organs dry weight for Sugar beet type and minimum value was obtained for treating both types of Spinach without using fertilizer. Studying leaf area index trend and growth rate diagrams indicate that Nano fertilizer has a positive effect on all plant growth steps.