In this research study, biodiesel was produced via transesterification process from the crude oil of milk bush ("thevetia peruviana") with methanol as alcohol. Two molar ratio 1:6 and 1:10 of oil to methanol was used to determine the conversion rate at two different temperature 45 and 60℃. Sodium hydroxide was used as catalyst at. The experiment was carried out at atmospheric pressure. The reaction was carried out in a batchtype reaction vessel. The results obtained shows that a maximum conversion of 94% of oil to methyl ester was achieved at 60ºC with molar ratio 1:10 of oil to methanol. The biodiesel produced agrees with ASTM and German standards.