The present study is an attempt to investigate the impact of mysticism on Safavid dynasty architecture. Moreover, the effect of mysticism on Mosque of Sheikh Lutfallah in Isfahan was explored which is recognized as one of the rarest and the biggest architectural manifestation of Safavid dynasty. It is a fact that architecture is inseparable from mysticism. This association between religions and the indigenous art is yet more apparent in Islam. So, it can be reasoned that mysticism lies in the essence of all religions. Since religion owns no instrument more effective and an exegete better than art especially architecture of temples and mosques to be introduced, as architecture found no stronger supporter than Islam. This issue becomes evident when Mosque of Sheikh Lotfollah of Isfahan architecture is taken into consideration. Accordingly, the present study aims through documentary and direct observation examines the significant of spiritual art and manifestation of mysticism in Safavid dynasty architecture in general and in Mosque of Sheikh Lotfollah, Isfahan in specific. Hence, the forgotten beauties would be reminded and the role of mysticism in directing art and artist to their divine destination is specified.