Chromosomal nif Genes Transfer by Conjugation in Nitrogen Fixing Azotobacter chroococcum to Lactobacillus plantarium

Adel Kamal Khider ； Aras Muhammad khidher

《Current Research Journal of Biological Sciences》 3卷2期 (2011/03) Pp. 155-164

並列摘要

To determine the possibility of transferring chromosomal nitrogen fixation genes (nif genes) from Azotobacter chroococcum to Lactobacillus planetarium, a total of 72 Azotobacter chroococcum isolated from Erbil governorate, Iraq were culturally, morphologically and biochemically characterized. Genes for atmospheric nitrogen fixation, located on the chromosome of Azotobacter chroococcum isolates were transferred by conjugation process to a recipient Lactobacillus plantarium isolated from Erbil city soils. The chromosomal genes transferred were verified by analysis of the genomes of donor, recipient and putative transconjugants, by polymorphism of DNA bands obtained through amplification of nifH1, nifH2, nifH3, nifU and nifV genes by PCR technique. The transconjugant cells promote an efficient fixation of nitrogen in liquid cultures fixed 0.2% nitrogen, and in the soil as inoculums of wheat plants, fixed 0.31% nitrogen and solublized 11.71 ppm phosphorus, beside all advantages of Lactic acid bacteria, and probably to be used as inoculums for both nitrogen fixation and solublizing insoluble phosphorus components, and used as biofertilizers

並列關鍵字

Azotobacter chroococcum ； conjugation and gene transfer ； Lactobacillus plantarium ； nif genes ； nitrogen fixation