Genetic Relationships of two Sonchus Species Collected from two Locations in Khartoum State Using RAPD Markers

H. H. Elkamali ； M. S. M. El-Kheir ； R. S. Habeballa ； N. B. Hamaza ； I. E. Abdalla ； E. I. Ahmedani ； A. A. S. Mohammed ； B. A. A. Abuzaid ； M. B. Mohammed ； T. Y. Ahmed

《Current Research Journal of Biological Sciences》 3卷2期 (2011/03) Pp. 95-99

並列摘要

Random amplified polymorphic DNA markers were used to assess genetic diversity in Sonchus oleraceus and Sonchus cornatus (Asteraceae), vulnerable medicinal plants collected from two locations (Shambat and Omdurman) in Khartoum state. The DNA extracted by Cetyltrimethyl ammonim bromide method (CTAB) from the plant sample was amplified by successive PCR cycles. Using 15 random primers, the amplified DNA was subjected to agarose gel electrophoresis. 74 different bands were observed under UV light. A total of 51 polymorphisms (3.4 polymorphic markers per primer) out of 74 (4.9 fragments per primer) were obtained from the 15 primers used. Unweighted Pair Group Method with Arithmetic Averages (UPGMA) clustering resulted in two major groups. According to the dendrogram, two groups were obtained based on genetic distance. The first group is indicated by A1 and A2 which were genetically similar. The second group included B1 and B2. S. oleraceus and S. cornatus from Shambat area clustered together on dendrogram and those from Omdurman area in other cluster.

並列關鍵字

RAPD fingerprinting ； genetic diversity ； Sonchus spp. ； Sudan