Expression Analysis of CAMP-Responsive Element Modulator Mrna in Different Tissues of Goat

Nasim Javdan ； Jasem Estakhr

《Current Research Journal of Biological Sciences》 4卷1期 (2012/01) Pp. 27-32

並列摘要

CAMP-Responsive Element Modulator (CREM) is a key factor in the regulation of the expression of number of post-meiotic genes during spermatogenesis. CREM binds to transcription factor and regulates gene expression in spermatids. In the present study tissue-specific expressions of CREM and different development stage expressions of CREM in testes of goat were quantified using qRT-PCR. The highest level was detected in testes (p＜0.05). The expression of CREM mRNA reached the highest level in 20-wk-old testes (p＜0.05). Furthermore, immunohistochemistry localized CREM mainly expressed in round spermatids, but not in spermatogonium and spermatocyte. These results indicated that CREM was a regulation proteins and it may play an important role in spermatid maturation.

並列關鍵字

CAMP-responsive element modulator ； gene expression ； goat ； QRT-PCR