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Evaluation of Water and Ethanol Extract of Eucalyptus globulus Labillardiere (Myrtaceae) Leaves against Immature Stages of Filarial Vector Culex quinquefasciatus Say (Diptera: Culicidae)
並列摘要
Aim of the present study was to evaluate larvicidal and pupicidal effect of aqueous and ethanol extract of Eucalyptus globulus leaves against immature stages of Culex mosquitoes. The experiment was conducted in the Botany Laboratory, University of Gondar, Gondar from February 2011 to May 2011. The standard WHO protocol was used to check five concentrations such as 100, 250, 500, 750 and 1000 ppm against II, III, IVth instar larvae and pupae of Culex mosquitoes, respectively. Irrespective of the concentrations tested and the period of exposure, 100% larval mortality was recorded in 1000 ppm concentration after 72 h exposure period followed by 96% in 750 ppm and 90% in 500 ppm. The percentage of larval mortality was based on concentration of the extract and the exposure period. When both are increased the percentage of larval mortality was also increased. After 72 h, 100% pupal mortality was recorded in 500, 750 and 1000 ppm concentration. Study reveled that aqueous and ethanol extract of Eucalyptus leaves is having strong insecticidal activity and it could be useful for the control of larval and pupal stage of Culex mosquitoes in small breeding places. Further, effective simple formulation and field evaluation of this plant extract is suggested for successful implementation of large scale use in mosquito control program.