- 期刊
- OpenAccess
All Serotypes of Dengue Viruses Circulating in Kuala Lumpur, Malaysia
並列摘要
Dengue is a severe disease caused by dengue virus (DENV), transmitted to human being by infected Aedes mosquitoes. It is a major public health concern in Southeast Asia due to its fatality in the form of Dengur Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). The objective of the study was to isolate and identify dengue virus serotypes prevalent in endemic areas of Kuala Lumpur and Selangor in Malaysia by virus culture, indirect immunoflurecent assay and molecular techniques. A total number of 232 sera samples were obtained from patients with clinical manifestations of dengue fever reported to University Kebangsaan Malaysia Medical Centre (UKMMC). The sera samples collected, were analyzed for IgM/IgG detection for the assessment of primary and secondary dengue fever, propagation in cell-line C36/36, Indirect Immunoflurecent Assay (IFA) and RT-PCR. The study confirmed 46 dengue cases where 15 (32.61%) were dual infections with DENV-1 and DENV- 4, 12 (26.09%) dual infections with DENV-3 and DENV-4, and 11 (23.91%) were dual infection with DENV-2 and DENV-4. Only 1 (2.17%) was dengue infection with DENV-3 and 7 (15.22%) were with DENV-4. Dengue serotype 4 was the most common serotype identified in the present study .The highest number of dengue cases detected in Cheras, Kuala Lumpur where all 4 types of dengue virus were prevalent. All serotypes of dengue viruses circulation only in Kuala Lumpur and Selangor Malaysia, needs further strengthening of the dengue preventive measure in the city areas and in the country.