In this study Eryngium maritimum roots were collected from plants grown at Tlemcen in west Algeria. They were powdered and extracted why to assess, the one hand, total phenolic and flavonoid content, on the other hand, antioxidant, antibacterial and antifungal activities of (methanol extract, acetone extract, ethyl acetate fraction and butanol fraction). The results showed that the amount of total phenolic and flavonoid content was higher in acetone extract (55.80±2.75 mg PE/g DW, 1.505±0.013 mg RE/g DW) than methanol extract (46.72±4.69 mg PE/g DW, 1.138±0.016 mg RE/g DW). Higher Free Radical Scavenging and iron reducing power of butanol extract was observed (0.0104±0.0004 and 0.1139±0.0112 mg/mL). Results of antibacterial activity showed that Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Bacillus cereus were inhibited by all the extract. Thus, only methanol and butanol extract was active against Pseudomonas aeruginosa. Results showed that ethyl acetate extract revealed the strongest activity against all the test fungi specially, toward Aspergillus flavus (10 mm at 50 mg/mL).