Allelopathic potential of wheat plant and genotypic variation among cultivars has been observed in several studies. In order to recognize and screen allelopathic potential of wheat genotypes against "Amaranthus retroflexus", "Hordeum spontaneum" and "Avena fatua", a glasshouse experiment was conducted, using 68 wheat genotypes. Root and shoot water extracts of wheat genotypes were prepared from fourth leaf stage wheat plants. Weeds root length was measured after germination in Petri dishes exposed to wheat extracts. The results showed considerable variation in inhibitory potential of wheat genotypes. Shoot extracts had higher allelopthic effects than root extract. The highest inhibitory effects were observed in the cultivars Rasool, Mahdavi, Navid, Karaj1, Adl1, Gaspard, Gascogen, Bam, Arvand, Baiat and Azadi, but high variation in weed responses were observed. In conclusion, some wheat genotypes had more allelopathic potential so they could be built into weed management programs.