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Genetic Relationships among ＂Aegilops＂ L. and Triticum L. Species based on the Internal Transcribed Spacer Sequences of nrDNA (ITS)
並列摘要
Studying of genetic relationships among ＂Triticum L.＂ and ＂Aegilops L.＂ species by direct sequencing of PCR-amplified Internal Transcribed Spacer (ITS) of nuclear ribosomal DNA to investigate the polymorphism in nucleotide sequences among 19 ＂Aegilops L.＂ and 7 ＂Triticum L.＂ species. ITS sequences were aligned with CLUSTAL W 2.1 multiple sequence aligment program. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) and Neighbor-Joining (NJ) methods. ITS region ranged from 600 to 602 bp. The length of ITS1 was 221-222 bp and ITS2 was 215-217 bp. The 5.8S subunit was 163 bp long. The G+C content of the ITS1 region ranged from (60.3 to 63.9) % in all Triticum and Aegilops species. The G+C content of the 5.8S subunit was entirely identical in all species, 59.5%. The G+C content of the ITS2 region ranged from 59.9 to 63.5%. There were 67 variable sites (11.12%) in the entire ITS region. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) were discussed.