The antibiotic resistance of bacteria isolated from Clarias gariepinus from 3 farms in Ago-Iwoye, Nigeria was investigated. Morphological and biochemical characteristics of isolates revealed that majority of the bacteria belonged to the family Enterobacteriaceae. Staphylococcus aureus and Pseudomonas aeruginosa were also recovered. E. coli strains were highly resistant to ampicillin, chloramphenicol and oxytetracycline (82.4%). Majority of the Pseudomonas aeruginosa were resistant to ampicillin (63.6%), amoxycillin (54.5%), nalidixic acid (63.6%) and oxytetracycline (72.7%), whereas most of the Salmonella spp. were resistant to erythromycin (85.7%), gentamycin (71.4%), amoxicillin (57.1%), chloramphenicol (57.1%) and sulphamethoxazole (57.1%). All isolates were highly sensitive to ciprofloxacin, novobiocin and ofloxacin. While the presence of potentially pathogenic bacterial species as observed in the study may not present a serious human health hazard because of heat treatment accorded fish before consumption, the presence of antibiotic resistant strains should not be ignored because of the potential for horizontal gene transfer in the food chain.