In this study, microwave vacuum equipment was employed to dry lentinus edodes (Berk.) Sing. Response Surface Methodology was used to determine the optimal drying conditions. The significant levels were obtained by the equations. The optimal conditions for drying lentinus edodes (Berk.) Sing with microwave vacuum equipment were obtained as following: microwave power 2650W, lentinus edodes load 171 g. Under these optimum conditions, lentinus edodes sensory evaluation, lentinan content and drying time were 80, 4.33% and 11 min, respectively.