We studied water content, H_2O_2 content, antioxidant enzymes activities of different organs and root activity in potato cv. Desiree pretreated with NO under water-stress. It was found that the bound water content in root and root activity was significantly increased by treating NO before water-stress. Pretreatment with NO decreased the H_2O_2 content and raised activities of CAT and GR significantly in roots under water-stress. The results suggested that NO might enhance the resistance to water-stress in potato.