Flavones from bamboo leaves have special pharmacological effects and bioactivities. The extraction process was studied by microwave radiation technology through single factor test and orthogonal experiment design method to investigate the influence of experimental factors. The results indicated that optimum extraction conditions were concentration of ethanol 80% (v/v), weight ratio of solid/liquid 0.04 (w/w), extraction time 6 min, the extraction rate of flavones reaches maximum 3.2%.