The Objective of study is to investigate the effect of long-term supplement mixed cherry-carrot juice sports drink on long-distance runner physical performance. 22 subjects were randomly divided into two groups for two-phase cross, double-blind placebo-controlled trials, each stage of the intervention was four weeks and 4-week- Wash-out phase was between two intervention phases. Before and after 4 week crossover phase, Oxygen uptake dynamics and physical performance test was performed, before and after which the sample of blood and urine was collected. After intervention the average Watt and RPM of C group was higher than P group in 20 km test, the t of oxygen uptake kinetics of C group was faster than P group. After intervention, no group change of antioxidant capacity was observed between C and P beside T-AOC, whose group difference from pre-intervention to post-intervention was significant. The significant group difference of 8-isoPGF2a from pre-intervention to post-intervention was observed in post-exercise status. After long-term mixed cherry-carrot juice sports drink intervention, physical performance and oxygen uptake kinetics parameters are improved in long-distance runner. Total antioxidant capacity of plasma is increased; the changes of Oxidative stress injury markers are reduced.