Optimize the Extract of Polysaccharides from Chlorella pyrenoidosa Based on Inhibitory Effects of Saccharase

Dong-Yang Su ； Zhang Yang ； Gao-Fang Zhang ； Wei-Min Wang ； Yong-Jun Zhang ； Li-Yun Zhu

《Advance Journal of Food Science and Technology》 10卷1期 (2016/01) Pp. 53-58

並列摘要

In this study, response surface methodology was employed to optimize the extraction process of Crude Polysaccharides from Chlorella pyrenoidosa (CCPS) on the activity of saccharase with maximum inhibition. Three independent and main variables, including extraction time (h), temperature (°C) and ratio of liquid to raw material (mL/g), which were of significance for the saccharase inhibition rate of CCPS were studied and the quadratic regression rotary combination design was based on the results of a single-factors test. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The best extraction conditions are as follows: extraction time 100 min, temperature 85°C, ratio of liquid to raw material 50 mL/g. Under the optimization conditions, the experimental saccharase inhibition rate of CCPS was 42.27%, which was well matched with the predictive inhibition rate of 41.78% and the yield of polysaccharide was 4.23%.

並列關鍵字

Chlorella pyrenoidosa ； polysaccharides ； response surface methodology ； saccharase