Flavones from Ginkgo leaves have special pharmacological effects and bioactivities. This study was focused on extraction process through single factor tests and orthogonal experiments method to investigate the influence of experimental factors on the yield of flavones. The results indicated that under microwave condition the optimal extraction conditions were concentration of ethanol 70% (v/v), weight ratio of liquid/solid 7: 1 (w/w), extraction time 7 min, the extraction percentage of flavones reaches maximum 82%.